Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex
Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.
© The Author(s) 2020
2020
2020-11-23 13:04:07
1033
Mycobacterium abscessus complex, nontuberculous mycobacteria, diagnosis
kompleks Mycobacterium abscessus, netuberkulozne mikobakterije, diagnostika
Springer Nature
Alina
Minias
70
Lidia
Żukowska
70
Jakub
Lach
70
Tomasz
Jagielski
70
Dominik
Strapagiel
70
Su-Young
Kim
70
Won-Jung
Koh
70
Heather
Adam
70
Ruth
Bittner
70
Sara
Truden
70
Marija
Žolnir-Dovč
70
Jarosław
Dziadek
70
UDK
4
579
ISSN pri članku
9
2045-2322
DOI
15
10.1038/s41598-020-73607-x
COBISS_ID
3
38347779
OceCobissID
13
18727432
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