20.500.12556/DiRROS-12541
Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps
Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed.
sinusitis
nasal polyps
Th1 cells
Th2 cells
Th17 cells
transcription factors
chronic rhinosinusitis
sinizutis
nosni polipi
celice Th1
celice Th2
celice Th17
transkripcijski faktorji
kronični rinosinuzitis
true
false
true
Association of Radiology and Oncology
Angleški jezik
Ni določen
© 2019 Tanja Kosak Soklic, Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar- Boltezar, Peter Korosec
Neznano
2020-10-09 14:24:01
2020-10-09 14:24:01
2022-08-18 03:38:00
0000-00-00 00:00:00
2019
0
Slovenija
0
Soavtorji: Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar-Boltezar, Peter Korosec;
Nasl. z nasl. zaslona;
Opis vira z dne 26. 7. 2019;
str. 323-330
iss. 3
Vol. 53
Sep. 2019
0000-00-00
Zaloznikova
Objavljeno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
616.21
1581-3207
10.2478/raon-2019-0029
2048522353
784507
RAZ_Soklic_Tanja_i2019.pdf
RAZ_Soklic_Tanja_i2019.pdf
1
D70A5366437474F51F17AE72616F3CCD
8d33ce970ff435cf28ae83150d647579bbf1d858f8b4d948855723f7986fc93d
88d214a8-17b6-11ed-b6b8-001a4af901a5
https://dirros.openscience.si/Dokument.php?lang=slv&id=17219
https://www.degruyter.com/downloadpdf/j/raon.ahead-of-print/raon-2019-0029/raon-2019-0029.pdf
1
https://dirros.openscience.si/Dokument.php?lang=slv&id=15241
Univerzitetna klinika za pljučne bolezni in alergijo Golnik
0
0
0