20.500.12556/DiRROS-12527 Expression of FGFR1-4 in malignant pleural mesothelioma tissue and corresponding cell lines and its relationship to patient survival and FGFR inhibitor sensitivity Malignant pleural mesothelioma (MPM) is a devastating malignancy with limited therapeutic options. Fibroblast growth factor receptors (FGFR) and their ligands were shown to contribute to MPM aggressiveness and it was suggested that subgroups of MPM patients could benefit from FGFR-targeted inhibitors. In the current investigation, we determined the expression of all four FGFRs (FGFR1-FGFR4) by immunohistochemistry in tissue samples from 94 MPM patients. From 13 of these patients, we were able to establish stable cell lines, which were subjected to FGFR1-4 staining, transcript analysis by quantitative RT-PCR, and treatment with the FGFR inhibitor infigratinib. While FGFR1 and FGFR2 were widely expressed in MPM tissue and cell lines, FGFR3 and FGFR4 showed more restricted expression. FGFR1 and FGFR2 showed no correlation with clinicopathologic data or patient survival, but presence of FGFR3 in 42% and of FGFR4 in 7% of patients correlated with shorter overall survival. Immunostaining in cell lines was more homogenous than in the corresponding tissue samples. Neither transcript nor protein expression of FGFR1-4 correlated with response to infigratinib treatment in MPM cell lines. We conclude that FGFR3 and FGFR4, but not FGFR1 or FGFR2, have prognostic significance in MPM and that FGFR expression is not suffcient to predict FGFR inhibitor response in MPM cell lines. malignant pleural mesothelioma fibroblast growth factor receptors azbestos immunotherapy chemotherapy genomic analysis infigratinib maligni plevralni mezoteliom receptorji za fibroblastne rastne dejavnike azbest imunoterapija kemoterapija genomska analiza infigratinib true false true MDPI Angleški jezik Ni določen © 2019 by the authors Neznano 2020-10-07 11:18:52 2020-10-07 11:18:53 2022-08-18 03:37:56 0000-00-00 00:00:00 2019 0 Švica 0 Soavtorji iz Slovenije: Katja Mohorcic, Tanja Cufer, Ales Rozman, Izidor Kern; Nasl. z nasl. zaslona; Opis vira z dne 17. 9. 2019; Št. prispevka: 1091; str. 1-14 iss. 9 Vol. 8 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2073-4409 10.3390/cells8091091 2048535409 519958809 RAZ_Vlacic_Gregor_i2019.pdf RAZ_Vlacic_Gregor_i2019.pdf 1 F0416A5D8E35B3782493059018061316 dda27d9ba524fde843b4db905a85de959578445504d34f7b6ddfd7d38f139e40 8cd580ef-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17295 https://www.mdpi.com/2073-4409/8/9/1091/pdf 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=15217 Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0