20.500.12556/DiRROS-12303 Genomic insights into the Mycobacterium kansasii complex : an update Only very recently, has it been proposed that the hitherto existing Mycobacteriumkansasii subtypes (I–VI) should be elevated, each, to a species rank. Consequently, the former M. kansasii subtypes have been denominated as Mycobacterium kansasii (former type I), Mycobacterium persicum (II), Mycobacterium pseudokansasii (III), Mycobacterium innocens (V), and Mycobacterium attenuatum (VI). The present work extends the recently published findings by using a three-pronged computational strategy, based on the alignment fraction-average nucleotide identity, genome-to-genome distance, and core-genome phylogeny, yet essentially independent and much larger sample, and thus delivers a more refined and complete picture of the M. kansasii complex. Furthermore, five canonical taxonomic markers were used, i.e., 16S rRNA, hsp65, rpoB, and tuf genes, as well as the 16S-23S rRNA intergenic spacer region (ITS). The three major methods produced highly concordant results, corroborating the view that each M. kansasii subtype does represent a distinct species. This work not only consolidates the position of five of the currently erected species, but also provides a description of the sixth one, i.e., Mycobacterium ostraviense sp. nov. to replace the former subtype IV. By showing a close genetic relatedness, amonophyletic origin, and overlapping phenotypes, our findings support the recognition of the M. kansasii complex (MKC), accommodating all M. kansasii-derived species and Mycobacterium gastri. None of the most commonly used taxonomic markers was shown to accurately distinguish all the MKC species. Likewise, no species-specific phenotypic characteristics were found allowing for species differentiation within the complex, except the non-photochromogenicity of M. gastri. To distinguish, most reliably, between the MKC species, and between M. kansasii and M. persicum in particular, whole-genome-based approaches should be applied. In the absence of clear differences in the distribution of the virulence-associated region of difference 1 genes among the M. kansasii-derived species, the pathogenic potential of each of these species can only be speculatively assessed based on their prevalence among the clinically relevant population. Large-scale molecular epidemiological studies are needed to provide a better understanding of the clinical significance and pathobiology of the MKC species. The results of the in vitro drug susceptibility profiling emphasize the priority of rifampicin administration in the treatment of MKC-induced infections, while undermining the use of ethambutol, due to a high resistance to this drug. Mycobacteriumkansasii complex Mycobacteriumostraviense sp. nov. non-tuberculous mycobacteria (NTM) whole genome sequencing taxonomy kompleks Mycobacterium kansasii Mycobacterium ostraviense sp. nov. netuberkulozne mikobakterije sekvenciranje genoma taksonomija true false true Frontiers Media SA Angleški jezik Ni določen 2020 Jagielski, Borówka, Bakuła, Lach, Marciniak, Brzostek, Dziadek, Dziurzy´nski, Pennings, van Ingen, Žolnir-Dovč and Strapagiel. Neznano 2020-07-31 13:16:59 2020-07-31 13:17:00 2022-08-18 03:36:32 0000-00-00 00:00:00 2020 0 Švica 0 Nasl. z nasl. zaslona; Soavtorica iz Slovenije: Manca Žolnir-Dovč; Opis vira z dne 17. 1. 2020; Št. prispevka: 2918; str. 1-27 Vol. 10 15 Jan. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1664-302X 10.3389/fmicb.2019.02918 2048616049 4146296 RAZ_Jagielski_Tomasz_i2020.pdf RAZ_Jagielski_Tomasz_i2020.pdf 1 72760826176C55EC74AF72C64D1E9033 e971753d6a5ae5b5809488ee6c4d96e28babf44a32e45bf1f989d790ad3b804c 8e33f121-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17317 RAZ_Jagielski_Tomasz_i2020.zip RAZ_Jagielski_Tomasz_i2020.zip 1 ED88A6C0856550F1756A977BD7ABB53C d1486082f108eaafedfc5411bd37afbceccf185e92212e0f9bbec000d12f02cc 8e46f36b-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17318 https://www.frontiersin.org/articles/10.3389/fmicb.2019.02918/full 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=14827 Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0