20.500.12556/DiRROS-11205 Ecological time series and integrative taxonomy unveil seasonality and diversity of the toxic diatom Pseudo-nitzschia H. Peragallo in the northern Adriatic Sea Pseudo-nitzschia H. Peragallo (1900) is a globally distributed genus of pennate diatoms that are important components of phytoplankton communities worldwide. Some members of the genus produce the neurotoxin domoic acid, so regular monitoring is in place. However, the identification of toxic members in routine samplings remains problematic. In this study, the diversity and seasonal occurrence of Pseudo-nitzschia species were investigated in the Gulf of Trieste, a shallow gulf in the northern Adriatic Sea. We used time series data from 2005 to 2018 to describe the seasonal and inter-annual occurrence of the genus in the area and its contribution to the phytoplankton community. On average, the genus accounted for about 15 % of total diatom abundance and peaked in spring and autumn, with occasional outbreaks during summer and large inter-annual fluctuations. Increased water temperature and decreased salinity positively affected the presence of some members of the genus, while strong effects could be masked by an unsuitable definition of the species complexes used for monitoring purposes. Therefore, combining morphological (TEM) and molecular analyses by sequencing the ITS, 28S and rbcL markers, eight species were identified from 83 isolated monoclonal strains: P. calliantha, P. fraudulenta, P. delicatissima, P. galaxiae, P. mannii, P. multistriata, P. pungens and P. subfraudulenta. A genetic comparison between the isolated strains and other strains in the Mediterranean was carried out and rbcL was inspected as a potential barcode marker in respect to our results. This is the first study in the Gulf of Trieste on Pseudo-nitzschia time series from a long-term ecological research (LTER) site coupled with molecular data. We show that meaningful ecological conclusions can be drawn by applying integrative methodology, as opposed to the approach that only considers species complexes. The results of this work will provide guidance for further monitoring efforts as well as research activities, including population genetics and genomics, associated with seasonal distribution and toxicity profiles. Pseudo-nitzschia morphology phylogeny seasonality time series Adriatic Sea ekologija morfologija filogenija Jadransko morje true false true Elsevier Angleški jezik Ni določen Neznano 2020-03-18 16:47:50 2020-03-18 16:47:51 2024-07-23 03:55:51 0000-00-00 00:00:00 2020 0 0 str. 1-20 101773 Vol. 93 Mar. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 574 1568-9883 10.1016/j.hal.2020.101773 40469253 512209945 https://www.sciencedirect.com/science/article/pii/S1568988320300536 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=13312 RAZ_Turk_Dermastia_Timotej_2020.pdf RAZ_Turk_Dermastia_Timotej_2020.pdf 1 779357ADDF115E92933AF7F4B6C2D56C 49d439fc21627c2eefcf7c06d5ddaad287f7ad46d1365e9e0ed55ccf9b9be376 1b583b08-4804-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27091 Nacionalni inštitut za biologijo 0 0 0