Influence of adipose-derived stromal vascular fraction on resorption of a large-volume free-fat transplant evaluated using T3D optical scanningKoren, Matic (Avtor) Kranjc Brezar, Simona (Avtor) Dovšak, Tadej (Avtor) Serša, Gregor (Avtor) Kansky, Andrej (Avtor) Ihan Hren, Nataša (Avtor) lipofillingmurine modelstromal vascular fractionBackground and Objectives: The main drawback of lipofilling is fat transplant volume loss, which makes long-term results unreliable. This study’s aim was to assess the influence of an adiposederived stromal vascular fraction (SVF) on volume retention in large-volume fat grafts. Materials and Methods: A murine model was used for the in vivo evaluation of fat-graft volume changes over 6 months. We used 28 immunocompromised nude NU(NCr)-Foxn1nu mice and human fat tissue as a liposuction by-product. Part of the fat tissue was used for SVF preparation. We created a fat transplant without SVF (SVF-) and with SVF (SVF+) groups. Large-volume grafts were injected above the sacrum and scapula in the same animal. Volume loss was evaluated using three-dimensional optical scanning at 14 days (T1), 3 months (T2), and 6 months (T3) after transplantation. Scans were processed with Artec Studio software to obtain stereolithography files. The volumes were calculated in RapidForm software 2006. Results: The highest volume loss was observed above the scapula at T3 (SVF- 85%; SVF+ 75%). There was a significant difference in volume between SVF-/SVF+ for grafts above the sacrum at T2, with lower loss in SVF+, and the significance became stronger at T3. The difference in volume loss was also significant above the scapula between SVF-/SVF+ at T3. Conclusions: Although we found a beneficial effect of SVF on the long-term survival of large-volume fat tissue transplants, volume loss due to other contributing factors was high.20232026-08-04 13:04:30Neznano31511UDK: 602ISSN pri članku: 2076-3417DOI: 10.3390/app13127100COBISS_ID: 155712515sl