HLA-DR–expressing fibroblast-like synoviocytes are inducible antigen presenting cells that present autoantigens in Lyme arthritisRouse, Joseph R. (Avtor) Danner, Rebecca (Avtor) Wahhab, Amanda (Avtor) Pereckas, Michaela (Avtor) Nguyen, Christine (Avtor) McClune, Mecaila E. (Avtor) Steere, Allen C. (Avtor) Strle, Klemen (Avtor) Jutras, Brandon L. (Avtor) Lochhead, Robert B. (Avtor) Lyme arthritisautoimmune diseasesinfectious diseaseObjective: HLA-DR–expressing fibroblast-like synoviocytes (FLS) are a prominent cell type in synovial tissue in chronic inflammatory forms of arthritis. FLS-derived extracellular matrix (ECM) proteins, including fibronectin-1 (FN1), contain immunogenic CD4+ T cell epitopes in patients with postinfectious Lyme arthritis (LA). However, the role of FLS in presentation of these T cell epitopes remains uncertain. Methods: Primary LA FLS and primary murine FLS stimulated with interferon gamma (IFNγ), Borrelia burgdorferi, and/or B burgdorferi peptidoglycan (PG) were assessed for properties associated with antigen presentation. HLA-DR–presented peptides from stimulated LA FLS were identified by immunopeptidomics analysis. OT-II T cells were co-cultured with stimulated murine FLS in the presence of cognate ovalbumin antigen to determine the potential of FLS to act as inducible antigen presenting cells (APCs). Results: FLS expressed HLA-DR molecules within inflamed synovial tissue and tendons from patients with postinfectious LA in situ. Major histocompatibility complex (MHC) class II and co-stimulatory molecules were expressed by FLS following in vitro stimulation with IFNγ and B burgdorferi and presented both foreign and self-MHC-II peptides, including an immunogenic T cell epitope derived from Lyme autoantigen FN1. Stimulated FLS induced proliferation of naive OT-II CD4+ T cells that were dependent on OT-II antigen and CD40. Stimulation with B burgdorferi PG enhanced FLS-mediated T cell activation. Conclusion: MHC-II+ FLS are inducible APCs that can induce CD4+ T cell activation in an antigen- and CD40-dependent manner. Activated FLS can also present ECM-derived Lyme autoantigens, implicating FLS in amplifying tissue-localized autoimmunity in LA.20242026-06-02 09:46:45Neznano29643UDK: 616.9ISSN pri članku: 2578-5745DOI: 10.1002/acr2.11710COBISS_ID: 279250691sl