CRISPR/dCas9-based systems: mechanisms and applications in plant sciencesSoong Karlson, Chou Khai (Avtor) Mohd-Noor, Siti Nurfadhlina (Avtor) Nolte, Nadja Franziska (Avtor) Tan, Boon Chin (Avtor) CRISPR interferenceCRISPR/dCas9 systemcrop improvementgene silencingRNAitranscriptional regulationRNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement.20212026-04-01 17:25:06Neznano28763UDK: 277.2ISSN pri članku: 2223-7747DOI: 10.3390/plants10102055COBISS_ID: 273252867sl