Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionVozelj, Anže (Avtor) Mahkovec Povalej, Tjaša (Avtor) Stare, Katja (Avtor) Tušek-Žnidarič, Magda (Avtor) Bačnik, Katarina (Avtor) Levak, Valentina (Avtor) Gutiérrez-Aguirre, Ion (Avtor) Podobnik, Marjetka (Avtor) Gruden, Kristina (Avtor) Coll Rius, Anna (Avtor) Lukan, Tjaša (Avtor) potato virus Ypotatocoat proteinsviral movementpoint mutationsPotato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY.20252025-12-18 11:14:00Neznano24784UDK: 577.2ISSN pri članku: 1098-5514DOI: 10.1128/jvi.01400-25COBISS_ID: 259503619sl