A prognostic human brain network for diffuse midline gliomaSidpra, Jai (Avtor) Lind, Valentina (Avtor) Cohen, Alexander L. (Avtor) Schaper, Frederic L. W .V. J. (Avtor) Stone, Thomas J (Avtor) Grabovska, Yura (Avtor) Biswas, Asthik (Avtor) Sudhakar, Sniya (Avtor) Sepulveda, Francisco (Avtor) Peres, Bruno S. (Avtor) Avsenik, Jernej (Avtor) diffuse midline gliomabrain networksprognosisbrain mappingDiffuse midline gliomas (DMGs) are near-universally lethal tumours of the childhood central nervous system1,2. In animal models, DMGs form brain-wide integrated networks through neuron-to-glioma synapses3-6 and glioma-to-glioma gap junctional coupling3. This extensive connectivity robustly promotes the growth and invasion of DMG3-9 and other glial malignancies10-12 through paracrine mechanisms and direct neuron-to-glioma synapses. However, the organization and clinical implications of these connections in the living human brain remain to be elucidated. Here, we develop tumour network mapping to compute the brain-wide connectivity profile of DMG, defining a conserved brain network across pontine and thalamic DMG associated with patient short-term survival (DMG network). Tumour functional connectivity with the DMG network was independently predictive of patient overall survival across two external validation cohorts. Tumour growth mapped to DMG network-specific trajectories and peak in-network neurometabolic changes across development spatiotemporally aligned with the peak age incidence of DMG. Analyses of single-nucleus RNA sequencing data confirmed diverse synaptic gene enrichment in high-connectivity DMG. Strikingly, incidental surgical resection of high-connectivity thalamic DMG tissue conferred a significant survival advantage. Collectively, these data define a conserved and prognostically important brain network in children with DMG, consistent with the hypothesis that DMGs exploit otherwise healthy brain circuits to promote tumour growth.20262026-07-27 14:00:42Neznano31315sl