Adipose tissue transcriptome profiles of local Krškopolje pig and modern hybrid pigs receving reduced protein diets:Poklukar Žnidaršič, Klavdija (Avtor) Škrlep, Martin (Avtor) Čandek-Potokar, Marjeta (Avtor) RNA sequencespigsadipose tissuehigh throughput sequencingRNAtranscriptome profilesgene expressionlocel breedshybrid breedsdatadata setThe main objective of this study was to investigate transcriptomic differences in subcutaneous adipose tissue between the local Krškopolje pig and modern hybrids, and to assess the effects of a reduced-protein diet in both genotypes. Comparative analysis between Krškopolje pigs and modern crossbreeds revealed 375 differentially expressed genes, with 189 upregulated and 186 downregulated in Krškopolje pigs. The upregulated genes were enriched in processes related to adipogenesis (SLC7A10, ADIRF, INHBB, SFRP2), extracellular matrix remodeling (COL6A5, COL4A5, COL2A1), calcium signaling (TRPV4, CAMK2A, CALML5), pro-inflammatory cytokines (IL1A, TNFSF9, CXCL8, PTGS2), and cholesterol metabolism (CYP1A1, CYP2A19, CES1). In contrast, the reduced-protein diet induced only minor transcriptional changes at the individual gene level in both Krškopolje pigs and modern crossbreeds.Gene Expression Omnibus (GEO)20252026-03-20 11:21:09Neznano28462sl