Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean eventsJelenčič, Amadej (Avtor) Štebih, Dejan (Avtor) Demšar, Tina (Avtor) Dobnik, David (Avtor) genetically modified (GM) cropstransgenicsoybeanGlycine max (L.) merrquantificationdigital PCR (dPCR)multiplexmulti-targetPlant genetic engineering represents an important aspect of modern agriculture, and new geneticallymodified (GM) crop varieties are entering the market on a regular basis. This necessitates thedevelopment of high throughput multi-target analytical methods to detect and quantify theirpresence for regulatory compliance. In this study, we present a multiplex dPCR method for discrimi-native quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of theplatform’s multiple fluorescence detection channels. The assays complied with the minimum perfor-mance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range,making them suitable for use in routine detection and quantification of GM crops. This methodrepresents the most comprehensive multi-target GM soybean quantification approach to date with-out the need for prior screening and features a simplified workflow, making it suitable for widespreadadoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCRGM crop quantification assays to address the evolving demands of regulatory monitoring.20262026-03-19 09:34:05Neznano28436sl