Microalgal communities in mucilage aggregates (Northern Adriatic Sea, summer 2024) based on microscopy and metabarcodingUbaldi, Marika (Avtor) Neri, Francesca (Avtor) Montali, Giorgia (Avtor) Romagnoli, Tiziana (Avtor) Tomasini, Aurora (Avtor) Cerino, Federica (Avtor) Turk Dermastia, Timotej (Avtor) Mozetič, Patricija (Avtor) Francé, Janja (Avtor) Spoto, Camilla (Avtor) Accoroni, Stefano (Avtor) Totti, Cecilia (Avtor) mucilageNorthern Adriatic SeaphytoplanktonmicroalgaemetabarcodingmicroscopyGonyaulax fragilisHarmful Algal BloomsThe mucilage phenomenon consists of the appearance of large gelatinous aggregates floating in the water column. In summer 2024, this event has reappeared in the Northern Adriatic Sea (NAS) on a large scale. This study provides an integrated characterization of the microalgal community within mucilage aggregates and surrounding waters in two NAS areas (Gulf of Trieste and Conero Riviera) using light microscopy (LM), metabarcoding (MB) based on ribosomal 18S V4 and V9 markers, and scanning electron microscopy (SEM) for selected taxa identification. Mucilage aggregates acted as dynamic microbial hotspots, hosting a rich diatom community, with abundances 1–2 orders of magnitude higher than in the surrounding water. Dominant diatom species were Cylindrotheca closterium, Nitzschia spp., Nitzschia gobbii, and Thalassionema nitzschioides. Some phytoflagellates (e.g., Tetraselmis spp.) and dinoflagellates (e.g., Karlodinium veneficum, Pseliodinium fusus, and Wangodinium sinense) were detected exclusively by MB, while LM and SEM revealed species missed by other approaches. Gonyaulax fragilis, one of the species indicated as an important mucus producer, was present at the onset and throughout the phenomenon, as detected by LM and MB. The integrated approach improves knowledge of microalgal communities in NAS mucilage.20262026-01-27 13:53:42Neznano25699sl