Nontargeted urinary profiling strategy for endocrine-disrupting chemicals in women with ovarian malignanciesPlešnik, Helena (Avtor) Rekar, Žan (Avtor) Stevanović, Stefanela (Avtor) Virant-Klun, Irma (Avtor) Imamović-Kumalić, Senka (Avtor) Sladič, Mateja (Avtor) Mazej, Darja (Avtor) Snoj Tratnik, Janja (Avtor) Horvat, Milena (Avtor) Kosjek, Tina (Avtor) ovarian cancerbiomarkersexposurenontargeted screeninghigh-resolution mass spectrometryanalytical coveragereversed-phaseHILICEndocrine-disrupting chemicals (EDCs), includingknown and unknown parent compounds, their metabolites, andtransformation products, are pervasive in daily life, posingincreasing risks to human health and the environment. Thisstudy employed a high-resolution mass spectrometry-basednontargeted screening approach, integrating polar (HILIC) andreversed-phase separations to expand the chemical space coverageand, supported by open-science tools and resources, evaluatedurinary chemical profiles to assess internal EDC exposure. Among106 annotated biomarkers of exposure, six exhibited significantlyhigher normalized intensities in patients with ovarian malignanciescompared to healthy controls (p < 0.05). This suggests theirgreater exposure to phthalates (diethylhexyl phthalate and diethylphthalate), pesticides (metolachlor metabolite and 4-nitrophenol), a UV filter (benzophenone-1), and an industrial byproduct (4-methyl-2-nitrophenol). These compounds may interfere with hormonal regulation, potentially contributing to cancer development.While these findings highlight potential differences in internal EDC exposure, the study primarily demonstrates the applicability ofnontargeted urinary profiling for chemical exposure assessment. By providing new insights into EDCs burden and its pathologicalimplications, this work contributes to advancing next-generation chemical risk assessment within the European Partnership for theAssessment of Risks from Chemicals initiative and supports the development of preventive strategies to mitigate environmentalcancer risks20252025-12-02 14:22:31Neznano24499sl