Synthesis of a water-soluble BODIPY for targeting and assessing the function of endoplasmic reticulumTricomi, Jacopo (Avtor) Biagiotti, Giacomo (Avtor) Chastel, Tommy (Avtor) Filiberti, Serena (Avtor) Kokot, Hana (Avtor) Mancusi, Francesca (Avtor) Žežlina, Maja (Avtor) Rajeh, Layal (Avtor) Urbančič, Iztok (Avtor) Bodin, Stéphane (Avtor) BODIPYSonogashiracross-couplingazide-alkyne cycloadditionendoplasmic reticulumorganelle labelingchick chorioallantoic membrane modelWe report here on a straightforward methodology to synthesize a new water-soluble fluorescent probe TrisBODIPY-OH 1 that contains three pH-independent hydrophilic arms. This probe has been prepared by exploiting a synthetic strategy that includes as a key step the combination of a Cu(I)-catalyzed azide−alkyne cycloaddition (CuAAC) and a Sonogashira cross-coupling in a sequential one-pot approach. Tris-BODIPY-OH 1 provides a significant advancement in the field by expanding the BODIPY toolbox with a biocompatible water-soluble probe, which can be used to specifically label and assess the function of the endoplasmic reticulum.American Chemical Society 20252025-10-30 13:28:52Neznano23976ZDAsl© 2025 The Authors.