Agents of European beech declineOgris, Nikica (Avtor) Brglez, Ana (Avtor) Kavčič, Andreja (Avtor) Zajc Žunič, Janja (Avtor) De Groot, Maarten (Avtor) Piškur, Barbara (Avtor) research dataFagus sylvaticadeclinedamagecomplex diseasefungiinsectsPhytophthoraendophytesstressData collected in the project "Diseases, pests and drought stress of European beech at various climate change scenarios (V4-2026), Working package 2: Causes of the decline of European beech in Slovenia". Data was collected on 40 sampling plots, 39 plots on systematic grid 16 × 16 km and one additional plot in the Alps. On each sampling plot two trees were felled, altogether 80 trees: one visually healthy, and one damaged tree. Visual assessment of crown condition and damaging agents was evaluated following methods of ICP Forests. Rhizosphere soil was collected from each sampled tree for the Phytophthora baiting assay. For fungal isolations five parts of a tree were sampled: leaves, twigs (up to 20 mm in diameter), branches (over 20 mm in diameter), trunk, surface roots and root collar (up to 25 cm from the ground). Four samples from each tree part were collected. Finally, 20 samples per tree and 40 samples per plot were collected, yielding 1.600 samples in total. The samples were surface sterilized. Four subsamples were collected from one sample, altogether, 6.400 subsamples/tissue pieces. Four subsamples were plated on potato dextrose agar supplemented with streptomycin. Fungi and Phytophthora species were identified using molecular techniques, while insects were identified based on their morphological characteristics.Slovenian Forestry Institute20252025-09-25 12:46:27Neznano23694sl