{"@context": "http://schema.org", "@id": "https://doi.org/10.5281/zenodo.18415123", "@type": "https://schema.org/Dataset", "identifier": "https://doi.org/10.5281/zenodo.18415123", "name": "In vitro genotoxicity assessment of graphene quantum dots using a 3D HepG2 model", "creator": [{"name": "Durmisevic, Irma", "givenName": "Irma", "familyName": "Durmisevic", "affiliation": [{"@type": "Organization", "name": "University of Sarajevo", "@id": "https://ror.org/02hhwgd43"}], "@id": "https://orcid.org/0009-0007-7386-0561", "@type": "Person"}, {"name": "Haveri\u0107, Anja", "givenName": "Anja", "familyName": "Haveri\u0107", "affiliation": [{"@type": "Organization", "name": "University of Sarajevo"}], "@id": "https://orcid.org/0000-0002-2398-3535", "@type": "Person"}, {"name": "Kolo\u0161a, Katja", "givenName": "Katja", "familyName": "Kolo\u0161a", "affiliation": [{"@type": "Organization", "name": "National Institute of Biology", "@id": "https://ror.org/03s5t0r17"}], "@id": "https://orcid.org/0000-0002-2855-2793", "@type": "Person"}, {"name": "Zegura, Bojana", "givenName": "Bojana", "familyName": "Zegura", "affiliation": [{"@type": "Organization", "name": "National Institute of Biology"}], "@id": "https://orcid.org/0000-0002-5731-0785", "@type": "Person"}], "author": [{"name": "Durmisevic, Irma", "givenName": "Irma", "familyName": "Durmisevic", "affiliation": [{"@type": "Organization", "name": "University of Sarajevo", "@id": "https://ror.org/02hhwgd43"}], "@id": "https://orcid.org/0009-0007-7386-0561", "@type": "Person"}, {"name": "Haveri\u0107, Anja", "givenName": "Anja", "familyName": "Haveri\u0107", "affiliation": [{"@type": "Organization", "name": "University of Sarajevo"}], "@id": "https://orcid.org/0000-0002-2398-3535", "@type": "Person"}, {"name": "Kolo\u0161a, Katja", "givenName": "Katja", "familyName": "Kolo\u0161a", "affiliation": [{"@type": "Organization", "name": "National Institute of Biology", "@id": "https://ror.org/03s5t0r17"}], "@id": "https://orcid.org/0000-0002-2855-2793", "@type": "Person"}, {"name": "Zegura, Bojana", "givenName": "Bojana", "familyName": "Zegura", "affiliation": [{"@type": "Organization", "name": "National Institute of Biology"}], "@id": "https://orcid.org/0000-0002-5731-0785", "@type": "Person"}], "publisher": {"@type": "Organization", "name": "Zenodo"}, "dateCreated": "2026-02-05T12:54:02.448848+00:00", "dateModified": "2026-03-19T16:35:55.347174+00:00", "datePublished": "2026-01-29", "contentSize": "26.18 KB", "size": "26.18 KB", "license": "https://creativecommons.org/licenses/by/4.0/legalcode", "description": "<p><span lang=\"EN-GB\">Graphene quantum dots (GQDs)</span><span lang=\"EN-GB\"> are nano-sized fragments of graphene sheets, with great potential for applications in optics, electrochemistry, and biomedicine, including drug delivery.</span></p>\n<p><span lang=\"EN-GB\">In this study, two types of GQDs were investigated: green-emitting (G-GQD) and blue-emitting (B-GQD) and their genotoxic potential was examined on the protein level. Flow cytometric analysis of &gamma;H2AX, a marker of DNA double-strand breaks, and phosphorylated histone H3 (p-H3), a marker of aneugenic activity, was performed in HepG2 spheroids after 24 hours of exposure to G-GQDs and B-GQDs.</span></p>\n<p><span lang=\"EN-GB\">HepG2 spheroids were exposed to graded concentrations of B- and G-GQD (12.5, 25, 50, and 100 &mu;g/mL) for 24 hours, respectively. Spheroids were dissociated into single-cell suspensions, washed twice with 1x PBS and fixed in 4% PFA. For flow cytometric analysis, cells were labelled with anti-H2AX pS139 antibody and anti-histone H3 pS128 antibody. REA-APC and REA-PE controls were applied to exclude non-specific antibody binding. For each sample, 10,000 events were acquired using a MACSQuant Analyzer 10 flow cytometer and MACSQuantify&trade; software (Miltenyi Biotech, Germany). Raw data were exported from the MACSQuantify software and analysed with FlowJo V10 software (Becton Dickinson, New Jersey, USA). </span></p>\n<p><span lang=\"EN-GB\"><span>&nbsp;</span></span></p>", "funding": [{"funder": {"@type": "Organization", "@id": "00k4n6c32", "name": "European Commission"}, "name": "TWINNING FOR EXCELLENCE TO STRATEGICALLY ADVANCE RESEARCH IN CARCINOGENESIS AND CANCER (101079113)", "identifier": "00k4n6c32::101079113", "url": {"identifier": "https://cordis.europa.eu/projects/101079113", "scheme": "url"}}], "url": "https://zenodo.org/records/18415123", "distribution": [{"@type": "DataDownload", "contentUrl": "https://zenodo.org/api/records/18415123/files/RAW data flow cytometry.docx/content", "encodingFormat": "application/octet-stream"}], "container_title": "Archives of Toxicology"}