Journal of Molecular Biology
Volume 418, Issues 3–4, 4 May 2012, Pages 145-160
Cryo-EM Structure of the Archaeal 50S Ribosomal Subunit in Complex with Initiation Factor 6 and Implications for Ribosome Evolution
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Graphical Abstract
Research Highlights
► The 6.6-Å cryo-EM structure of the archaeal 50S ribosomal subunit in complex with aIF6. ► Highest-resolution cryo-EM reconstruction of an isolated ribosomal subunit. ► Conservation of archaeal and eukaryotic IF6 binding across kingdoms. ► Implications for functional interactions of IF6 with translation factors. ► Structural insight into the reductive evolution of the archaeal ribosome.
Abbreviations
IF6
initiation factor 6
EM
electron microscopy
3D
three-dimensional
PDB
Protein Data Bank
EDTA
ethylenediaminetetraacetic acid
Keywords
ribosome
IF6
cryo-EM
CCD detector
evolution
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Present addresses: V. Godinic-Mikulcic, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia; A. Crnkovic, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia.
Copyright © 2012 Elsevier Ltd.