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Naslov:Genomic insights into the Mycobacterium kansasii complex : an update
Avtorji:ID Jagielski, Tomasz (Avtor)
ID Borówka, Paulina (Avtor)
ID Bakuła, Zofia (Avtor)
ID Lach, Jakub (Avtor)
ID Marciniak, Błażej (Avtor)
ID Brzostek, Anna (Avtor)
ID Dziadek, Jarosław (Avtor)
ID Dziurzyński, Mikołaj (Avtor)
ID Pennings, Lian (Avtor)
ID Ingen, Jakobus van (Avtor)
ID Žolnir-Dovč, Marija, Klinika Golnik (Avtor)
ID Strapagiel, Dominik (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (10,22 MB)
MD5: 72760826176C55EC74AF72C64D1E9033
 
.zip ZIP - Priloga, prenos (1,77 MB)
MD5: ED88A6C0856550F1756A977BD7ABB53C
 
URL URL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fmicb.2019.02918/full
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Only very recently, has it been proposed that the hitherto existing Mycobacteriumkansasii subtypes (I–VI) should be elevated, each, to a species rank. Consequently, the former M. kansasii subtypes have been denominated as Mycobacterium kansasii (former type I), Mycobacterium persicum (II), Mycobacterium pseudokansasii (III), Mycobacterium innocens (V), and Mycobacterium attenuatum (VI). The present work extends the recently published findings by using a three-pronged computational strategy, based on the alignment fraction-average nucleotide identity, genome-to-genome distance, and core-genome phylogeny, yet essentially independent and much larger sample, and thus delivers a more refined and complete picture of the M. kansasii complex. Furthermore, five canonical taxonomic markers were used, i.e., 16S rRNA, hsp65, rpoB, and tuf genes, as well as the 16S-23S rRNA intergenic spacer region (ITS). The three major methods produced highly concordant results, corroborating the view that each M. kansasii subtype does represent a distinct species. This work not only consolidates the position of five of the currently erected species, but also provides a description of the sixth one, i.e., Mycobacterium ostraviense sp. nov. to replace the former subtype IV. By showing a close genetic relatedness, amonophyletic origin, and overlapping phenotypes, our findings support the recognition of the M. kansasii complex (MKC), accommodating all M. kansasii-derived species and Mycobacterium gastri. None of the most commonly used taxonomic markers was shown to accurately distinguish all the MKC species. Likewise, no species-specific phenotypic characteristics were found allowing for species differentiation within the complex, except the non-photochromogenicity of M. gastri. To distinguish, most reliably, between the MKC species, and between M. kansasii and M. persicum in particular, whole-genome-based approaches should be applied. In the absence of clear differences in the distribution of the virulence-associated region of difference 1 genes among the M. kansasii-derived species, the pathogenic potential of each of these species can only be speculatively assessed based on their prevalence among the clinically relevant population. Large-scale molecular epidemiological studies are needed to provide a better understanding of the clinical significance and pathobiology of the MKC species. The results of the in vitro drug susceptibility profiling emphasize the priority of rifampicin administration in the treatment of MKC-induced infections, while undermining the use of ethambutol, due to a high resistance to this drug.
Ključne besede:Mycobacteriumkansasii complex, Mycobacteriumostraviense sp. nov., non-tuberculous mycobacteria (NTM), whole genome sequencing, taxonomy
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Kraj izida:Švica
Založnik:Frontiers Media SA
Leto izida:2020
Št. strani:str. 1-27
Številčenje:Vol. 10
PID:20.500.12556/DiRROS-12303 Novo okno
UDK:579
ISSN pri članku:1664-302X
DOI:10.3389/fmicb.2019.02918 Novo okno
COBISS.SI-ID:2048616049 Novo okno
Avtorske pravice:2020 Jagielski, Borówka, Bakuła, Lach, Marciniak, Brzostek, Dziadek, Dziurzy´nski, Pennings, van Ingen, Žolnir-Dovč and Strapagiel.
Opomba:Nasl. z nasl. zaslona; Soavtorica iz Slovenije: Manca Žolnir-Dovč; Opis vira z dne 17. 1. 2020; Št. prispevka: 2918;
Datum objave v DiRROS:31.07.2020
Število ogledov:1844
Število prenosov:1317
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Frontiers in microbiology
Skrajšan naslov:Front. microbiol.
Založnik:Frontiers Research Foundation
ISSN:1664-302X
COBISS.SI-ID:4146296 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:04.12.2019

Sekundarni jezik

Jezik:Ni določen
Ključne besede:kompleks Mycobacterium kansasii, Mycobacterium ostraviense sp. nov., netuberkulozne mikobakterije, sekvenciranje genoma, taksonomija


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