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Naslov:Genomic insights into the Mycobacterium kansasii complex : an update
Avtorji:Jagielski, Tomasz (Avtor)
Borówka, Paulina (Avtor)
Bakuła, Zofia (Avtor)
Lach, Jakub (Avtor)
Marciniak, Błażej (Avtor)
Brzostek, Anna (Avtor)
Dziadek, Jarosław (Avtor)
Dziurzyński, Mikołaj (Avtor)
Pennings, Lian (Avtor)
Ingen, Jakobus van (Avtor)
Žolnir-Dovč, Marija (Avtor)
Strapagiel, Dominik (Avtor)
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Only very recently, has it been proposed that the hitherto existing Mycobacteriumkansasii subtypes (I–VI) should be elevated, each, to a species rank. Consequently, the former M. kansasii subtypes have been denominated as Mycobacterium kansasii (former type I), Mycobacterium persicum (II), Mycobacterium pseudokansasii (III), Mycobacterium innocens (V), and Mycobacterium attenuatum (VI). The present work extends the recently published findings by using a three-pronged computational strategy, based on the alignment fraction-average nucleotide identity, genome-to-genome distance, and core-genome phylogeny, yet essentially independent and much larger sample, and thus delivers a more refined and complete picture of the M. kansasii complex. Furthermore, five canonical taxonomic markers were used, i.e., 16S rRNA, hsp65, rpoB, and tuf genes, as well as the 16S-23S rRNA intergenic spacer region (ITS). The three major methods produced highly concordant results, corroborating the view that each M. kansasii subtype does represent a distinct species. This work not only consolidates the position of five of the currently erected species, but also provides a description of the sixth one, i.e., Mycobacterium ostraviense sp. nov. to replace the former subtype IV. By showing a close genetic relatedness, amonophyletic origin, and overlapping phenotypes, our findings support the recognition of the M. kansasii complex (MKC), accommodating all M. kansasii-derived species and Mycobacterium gastri. None of the most commonly used taxonomic markers was shown to accurately distinguish all the MKC species. Likewise, no species-specific phenotypic characteristics were found allowing for species differentiation within the complex, except the non-photochromogenicity of M. gastri. To distinguish, most reliably, between the MKC species, and between M. kansasii and M. persicum in particular, whole-genome-based approaches should be applied. In the absence of clear differences in the distribution of the virulence-associated region of difference 1 genes among the M. kansasii-derived species, the pathogenic potential of each of these species can only be speculatively assessed based on their prevalence among the clinically relevant population. Large-scale molecular epidemiological studies are needed to provide a better understanding of the clinical significance and pathobiology of the MKC species. The results of the in vitro drug susceptibility profiling emphasize the priority of rifampicin administration in the treatment of MKC-induced infections, while undermining the use of ethambutol, due to a high resistance to this drug.
Ključne besede:Mycobacteriumkansasii complex, Mycobacteriumostraviense sp. nov., non-tuberculous mycobacteria (NTM), whole genome sequencing, taxonomy
Leto izida:2020
Založnik:Frontiers Media SA
Izvor:Švica
UDK:579
ISSN pri članku:1664-302X
OceCobissID:4146296 Povezava se odpre v novem oknu
COBISS_ID:2048616049 Povezava se odpre v novem oknu
DOI:10.3389/fmicb.2019.02918 Povezava se odpre v novem oknu
Opombe:Nasl. z nasl. zaslona; Soavtorica iz Slovenije: Manca Žolnir-Dovč; Opis vira z dne 17. 1. 2020; Št. prispevka: 2918;
Število ogledov:52
Število prenosov:36
Datoteke:URL URL - Predstavitvena datoteka, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fmicb.2019.02918/full
 
Nadgradivo:Front. microbiol.
Frontiers Research Foundation
 
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Avtorske pravice:2020 Jagielski, Borówka, Bakuła, Lach, Marciniak, Brzostek, Dziadek, Dziurzy´nski, Pennings, van Ingen, Žolnir-Dovč and Strapagiel.
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Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:04.12.2019

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