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PCR primers comparisons for a successful Tuber spp. DNA region amplification in routine identifications
Tina Unuk, Hojka Kraigher, Tine Grebenc, 2020

Povzetek: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS re-gion may, due to misidentification, mislabeling or nomen-clature errors in public databases, lead to incorrect or insuf-ficient identification, as is currently a case in the genus Tu b e r. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tu b e r species DNA region amplification. Thereby we (1) compared ampli-fication success for different Tu b e r species using fungal spe-cific primer pair ITS1f and ITS4 and (2) compared amplifi-cation success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aesti-vum. Based on results, Tuber aestivum was one of the most reluctant Tu b e r species in this study and in most cases failed to amplify with the above primer pair. After comparing dif-ferent ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for ampli-fication DNA region of T. ae stiv um as it resulted in high am-plification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus re-sulting in poor or no amplification success. Although prim-er pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tu b e r aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed.
Ključne besede: Tuber spp., ITS region, PCR amplification, ITS primers
DiRROS - Objavljeno: 30.07.2020; Ogledov: 35; Prenosov: 29
URL Celotno besedilo (0,00 KB)
Gradivo ima več datotek! Več...

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Jesenov ožig, Chalara fraxinea
Nikica Ogris, 2008

Ključne besede: jesenov ožig, Chalara fraxinea, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 25; Prenosov: 25
URL Celotno besedilo (0,00 KB)
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Smrekov lubadar močno uničuje gozdove na zgornjem Gorenjskem
Vida Papler-Lampe, 2008

Ključne besede: smreka, Picea abies, podlubniki, Gornjska, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 25; Prenosov: 23
URL Celotno besedilo (0,00 KB)
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Rjavenje borovih iglic, Mycosphaerella dearnessii
Dušan Jurc, 2008

Ključne besede: rjavenje iglic, iglice, bor, Pinus sp., Mycosphaerella dearnessii, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 26; Prenosov: 23
URL Celotno besedilo (0,00 KB)
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Gabrova pepelovka, Erysiphe arcuata
Tine Hauptman, 2008

Ključne besede: gabrova pepelovka, Erysiphe arcuata, beli gaber, Carpinus betulus, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 27; Prenosov: 27
URL Celotno besedilo (0,00 KB)
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Češnjeva listna pegavost, Blumeriella jaapii
Nikica Ogris, 2008

Ključne besede: češnjeva listna pegavost, Blumeriella jaapii, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 33; Prenosov: 25
URL Celotno besedilo (0,00 KB)
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Petrakovo rjavenje listja gorskega javora, Petrakia echinata
Tine Hauptman, 2008

Ključne besede: gorski javor, Acer pseudoplatanus, rjavenje listja, Petrakia echinata, bolezni drevja, gozdovi, varstvo gozdov
DiRROS - Objavljeno: 29.07.2020; Ogledov: 19; Prenosov: 19
URL Celotno besedilo (0,00 KB)
Gradivo ima več datotek! Več...

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