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Povzetek: Even though genetic resources represent a fundamental reservoir of options to achieve sustainable development goals in a changing world, they are overlooked in the policy agenda and severely threatened. The conservation of genetic resources relies on complementary in situ and ex situ approaches appropriately designed for each type of organism. Environmental and socioeconomic changes raise new challenges and opportunities for sustainable use and conservation of genetic resources. Aiming at a more integrated and adaptive approach, European scientists and genetic resources managers with long experience in the agricultural crop, animal and forestry domains joined their expertise to address three critical challenges: (1) how to adapt genetic resources conservation strategies to climate change, (2) how to promote in situ conservation strategies and (3) how can genetic resources conservation contribute to and benefit from agroecological systems. We present here 31 evidence-based statements and 88 key recommendations elaborated around these questions for policymakers, conservation actors and the scientific community. We anticipate that stakeholders in other genetic resources domains and biodiversity conservation actors across the globe will have interest in these crosscutting and multi-actor recommendations, which support several biodiversity conservation policies and practices.
Ključne besede: agroecology, climate change, in situ conservation, multi-actor engagement, policy
Objavljeno v DiRROS: 11.03.2024; Ogledov: 139; Prenosov: 58
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Povzetek: The aim of the study was to provide a comprehensive overview of global long-distance road transportation of industrial roundwood. The study focused on the maximum gross vehicle weight (GVW) limits allowed with different timber truck configurations, typical payloads in timber trucking, the road transportation share of the total industrial roundwood longdistance transportation volume, and the average long-distance transportation distances and costs of industrial roundwood. The study was carried out as a questionnaire survey. The questionnaire was sent to timber transportation logistics experts and research scientists in the 30 countries with the largest industrial roundwood removals in Europe, as well as selected major forestry countries in the world (Argentina, Australia, Brazil, Canada, Chile, China, Japan, New Zealand, South Africa, Türkiye, the United States of America and Uruguay) in February 2022, and closed in May 2022. A total of 31 countries took part in the survey. The survey illustrated that timber trucking was the main long-distance transportation method of industrial roundwood in almost every country surveyed. Road transportation averaged 89% of the total industrial roundwood long-distance transportation volume. Timber truck configurations of 4 to 9 axles with GVW limits of around 30 tonnes to over 70 tonnes were most commonly used. The results indicated that higher GVW limits allowed significantly higher payloads in timber trucking, with the lowest payloads at less than 25 tonnes, and the highest payloads more than 45 tonnes. The average road transportation distance with industrial roundwood was 128 km, and the average long-distance transportation cost in timber trucking was €11.1 per tonne of timber transported. In the entire survey material, there was a direct relationship between transportation distance and transportation costs and an inverse relationship between maximum GVW limits and transportation costs. Consequently, in order to reduce transportation costs, it is essential to maximise payloads (within legal limits) and minimise haul distances. Several measures to increase cost- and energy-efficiency, and to reduce greenhouse gas emissions in road transportation logistics, are discussed in the paper. On the basis of the survey, it is recommended that upto-date statistical data and novel research studies on the long-distance transportation of industrial roundwood be conducted in some countries in the future.
Ključne besede: timber logistics, timber hauling, timber trucking, gross vehicle weight, payload, transportation distance, transportation cost, cost efficiency, limit payload
Objavljeno v DiRROS: 28.02.2024; Ogledov: 120; Prenosov: 56
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Povzetek: The ecological impacts of long-term (press) disturbance on mechanisms regulating the relative abundance (i.e., commonness or rarity) and temporal dynamics of species within a community remain largely unknown. This is particularly true for the functionally important arbuscular mycorrhizal (AM) fungi; obligate plant-root endosymbionts that colonize more than two-thirds of terrestrial plant species. Here, we use high-resolution amplicon sequencing to examine how AM fungal communities in a specific extreme ecosystem—mofettes or natural CO2 springs caused by geological CO2 exhalations—are affected by long-term stress. We found that in mofettes, specific and temporally stable communities form as a subset of the local metacommunity. These communities are less diverse and dominated by adapted, “stress tolerant” taxa. Those taxa are rare in control locations and more benign environments worldwide, but show a stable temporal pattern in the extreme sites, consistently dominating the communities in grassland mofettes. This pattern of lower diversity and high dominance of specific taxa has been confirmed as relatively stable over several sampling years and is independently observed across multiple geographic locations (mofettes in different countries). This study implies that the response of soil microbial community composition to long-term stress is relatively predictable, which can also reflect the community response to other anthropogenic stressors (e.g., heavy metal pollution or land use change). Moreover, as AM fungi are functionally differentiated, with different taxa providing different benefits to host plants, changes in community structure in response to long-term environmental change have the potential to impact terrestrial plant communities and their productivity
Ključne besede: arbuscular mycorrhiza, elevated CO2, long-term experiments, soil biodiversity, soil hypoxia, next-generation sequencing, NGS
Objavljeno v DiRROS: 28.02.2024; Ogledov: 131; Prenosov: 63
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Povzetek: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Ključne besede: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Objavljeno v DiRROS: 02.02.2024; Ogledov: 419; Prenosov: 125
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Povzetek: Na 12 izbranih lokacijah v Mežiški dolini smo spomladi leta 2022 opravili popis epifitskih lišajev z uporabo slovenske metode SI in nemške metode VDI. Z obema metodama se ocenjuje kakovost zraka na podlagi obstoječih rastnih tipov steljk in vrst epifitskih lišajev, na katere vplivajo predvsem plinasta onesnažila v zraku. Dodatno smo določali tudi indekse toksitolerance. Skupaj smo na prostostoječem drevju evidentirali 34 vrst epifitskih lišajev; 25 v Zgornji Mežiški dolini in 27 vrst epifitskih lišajev v Spodnji Mežiški dolini. Na podlagi izračuna povprečnega indeksa toksitolerance za posamezno lokacijo smo ugotovili, da na vseh lokacijah prevladujejo vrste, ki so manj občutljive za onesnaževanje. Največji indeks zračne čistosti, določen po metodi VDI, je bil izračunan za lokacijo Lom, sledita lokaciji Žerjav in Tolsti vrh, kjer smo evidentirali tudi največje število vrst lišajev. Stanje lišajske vegetacije na vseh obravnavanih lokacijah je primerljivo in kaže, da so lokacije srednje obremenjene s plinastimi onesnažili. V ospredje lahko postavimo lokaciji Dolgo Brdo in Preški vrh, kjer je izpostavljenost plinastim onesnažilom velika do zmerna; na lokaciji Dolgo Brdo pa je bila tudi obrast lišajev revna. Izračunani indeksi zračne čistosti so sicer pokazali, da je kakovost zraka v Zgornji Mežiški dolini nekoliko boljša kot v Spodnji Mežiški dolini, vendar razlike niso statistično značilne.
Ključne besede: epifitski lišaji, kartiranje, bioindikatorji, onesnažen zrak, Mežiška dolina
Objavljeno v DiRROS: 25.01.2024; Ogledov: 629; Prenosov: 177
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