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Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon
Mojca Benčina, Apolonija Bedina Zavec, Damjana Drobne, Mitja Drab, Zala Jan, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021

Povzetek: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Ključne besede: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
DiRROS - Objavljeno: 25.01.2021; Ogledov: 1; Prenosov: 2
.pdf Celotno besedilo (1,88 MB)

Physical activity drops during summer holidays for 6- to 9-year-old children
Tadeja Volmut, Rado Pišot, Jurij Planinšec, Boštjan Šimunič, 2021

Povzetek: Regular physical activity (PA) reduces the health risk of childhood obesity and associated chronic diseases as well as mental health problems. Since PA declines as children age as well with future generations it is of highest importance to intervene in school and out-of-school settings. Out-of-school periods affect children's PA as it is mainly left to the interest and motivation of their parents. We compared accelerometer-based PA patterns in 93 6- to 9-year old children assessed four times: before (May/June), during (August), and after (September) summer holidays and at a 1-year follow up (May/June). Before summer holidays children were assessed also for anthropometry and motor tests. During summer holidays overall PA decreased by 18% (p < 0.001), physical inactivity increased by 5.5% (p < 0.001), moderate PA decreased by 53% (p < 0.001) and moderate to vigorous PA decreased by 45% (p < 0.001) when compared to before summer holidays. Furthermore, overall PA remained diminished also after summer holidays by 8.8% (p = 0.001) but recovered to baseline values at 1-year follow up. About 30% of overall PA and moderate to vigorous PA decrease during summer holidays could be explained by children's fitness level as a greater decrease was found in children with better results in standing long jump and 300-meter running time. Our finding detects an alarming summer holiday decrease in children PA that should not be neglected in future studies and intervention designs.
Ključne besede: MVPA, children, vacations, physical inactivity, sedentary behavior, accelerometer
DiRROS - Objavljeno: 19.01.2021; Ogledov: 13; Prenosov: 11
.pdf Celotno besedilo (416,68 KB)

The effect of olive fruit fly Bactrocera oleae (Rossi) infestation on certain chemical parameters of produced olive oils
Vasilij Valenčič, Bojan Butinar, Maja Podgornik, Milena Bučar-Miklavčič, 2021

Povzetek: Olives affected by active and damaging infestation (olive fruit fly Bactrocera oleae (Rossi)) were assayed for their chemical composition. Biophenols were determined by HPLC, sterols, triterpenic dialcohols, and fatty acids by gas chromatography analysis. The acquired data were statistically analyzed. Oils produced from %Istrska belica% fruit affected by active infestation compared to the oils made from fruit affected by damaging infestation showed higher amounts of total oleuropein biofenols (377.3 versus (vs.) 106.6 mg/kg), total biophenols (755 vs. 377 mg/kg), lignans (85.3 vs. 32.9mg/kg), the dialdehydic formof decarboxymethyl oleuropein aglycone (DMO-AgldA) (148.3 vs. 49.0 mg/kg), its oxidized form (DMO-Agl-dA)ox (35.2 vs. 8.5 mg/kg), the dialdehydic form of oleuropein aglycone (O-Agl-dA) (61.1 vs. 8.0 mg/kg), the dialdehydic form of ligstroside aglycone (L-Agl-dA) (63.5 vs. 28.0 mg/kg), the aldehydic form of oleuropein aglycone (O-Agl-A) (40.6 vs. 8.4 mg/kg), and lower amounts of tyrosol (Tyr) (6.0 vs. 13. 9 mg/kg) and the aldehydic form of ligstroside aglycone (L-Agl-A) (13.8 vs. 40.3 mg/kg). Higher values of stigmasterol (2.99%) and lower values of campesterol (2.25%) were determined in oils affected by damaging infestation; an increase in triterpenic dialcohols was also observed (3.04% for damaging and 1.62% for active infestation). Oils affected by damaging infestation, compared to active infestation, showed lower amounts of oleic acid (73.89 vs. 75.15%) and higher amounts of myristic (0.013 vs. 0.011%), linoleic (7.27 vs. 6.48%), and linolenic (0.74 vs. 0.61%) acids.
Ključne besede: olives, Istrska belica, olive fruit fly, biophenols, sterols, triterpenic dialcohols, fatty acids
DiRROS - Objavljeno: 13.01.2021; Ogledov: 38; Prenosov: 95
.pdf Celotno besedilo (4,01 MB)

Effects of home confinement on mental health and lifestyle behaviours during the COVID-19 outbreak : insights from the ECLB-COVID19 multicentre study
Saša Pišot, Rado Pišot, Boštjan Šimunič, Bassem Bouaziz, Liwa Masmoudi, Omar Boukhris, Hamdi Chtourou, Michael Brach, Khaled Trabelsi, Achraf Ammar, 2021

Povzetek: Although recognised as effective measures to curb the spread of the COVID-19 outbreak, social distancing and self-isolation have been suggested to generate a burden throughout the population. To provide scientific data to help identify risk factors for the psychosocial strain during the COVID-19 outbreak, an international cross-disciplinary online survey was circulated in April 2020. This report outlines the mental, emotional and behavioural consequences of COVID-19 home confinement. The ECLB-COVID19 electronic survey was designed by a steering group of multidisciplinary scientists, following a structured review of the literature. The survey was uploaded and shared on the Google online survey platform and was promoted by thirty-five research organizations from Europe, North Africa, Western Asia and the Americas. Questions were presented in a differential format with questions related to responses %before% and %during% the confinement period. 1047 replies (54% women) from Western Asia (36%), North Africa (40%), Europe (21%) and other continents (3%) were analysed. The COVID-19 home confinement evoked a negative effect on mental wellbeing and emotional status (P < 0.001; 0.43 % d % 0.65) with a greater proportion of individuals experiencing psychosocial and emotional disorders (+10% to +16.5%). These psychosocial tolls were associated with unhealthy lifestyle behaviours with a greater proportion of individuals experiencing (i) physical (+15.2%) and social (+71.2%) inactivity, (ii) poor sleep quality (+12.8%), (iii) unhealthy diet behaviours (+10%), and (iv) unemployment (6%). Conversely, participants demonstrated a greater use (+15%) of technology during the confinement period. These findings elucidate the risk of psychosocial strain during the COVID-19 home confinement period and provide a clear remit for the urgent implementation of technology-based intervention to foster an Active and Healthy Confinement Lifestyle AHCL).
Ključne besede: coronavirus, COVID-19, pandemic, public health, restrictions, isolation, home confinement, psychosocial health, mental wellbeing, depression, satisfaction, lifestyle, behaviours
DiRROS - Objavljeno: 18.12.2020; Ogledov: 110; Prenosov: 52
.pdf Celotno besedilo (732,17 KB)

Psychological consequences of COVID-19 home confinement : the ECLB-COVID19 multicenter study
Achraf Ammar, Patrick Mueller, Khaled Trabelsi, Hamdi Chtourou, Omar Boukhris, Liwa Masmoudi, Bassem Bouaziz, Michael Brach, Marlen Schmicker, Boštjan Šimunič, Rado Pišot, 2020

Povzetek: Background Public health recommendations and government measures during the COVID-19 pandemic have enforced restrictions on daily-living. While these measures are imperative to abate the spreading of COVID-19, the impact of these restrictions on mental health and emotional wellbeing is undefined. Therefore, an international online survey (ECLB-COVID19) was launched on April 6, 2020 in seven languages to elucidate the impact of COVID-19 restrictions on mental health and emotional wellbeing. Methods The ECLB-COVID19 electronic survey was designed by a steering group of multidisciplinary scientists, following a structured review of the literature. The survey was uploaded and shared on the Google online-survey-platform and was promoted by thirty-five research organizations from Europe, North-Africa, Western-Asia and the Americas. All participants were asked for their mental wellbeing (SWEMWS) and depressive symptoms (SMFQ) with regard to %during% and %before% home confinement. Results Analysis was conducted on the first 1047 replies (54% women) from Asia (36%), Africa (40%), Europe (21%) and other (3%). The COVID-19 home confinement had a negative effect on both mental-wellbeing and on mood and feelings. Specifically, a significant decrease (p < .001 and %% = 9.4%) in total score of the SWEMWS questionnaire was noted. More individuals (+12.89%) reported a low mental wellbeing %during% compared to %before% home confinement. Furthermore, results from the mood and feelings questionnaire showed a significant increase by 44.9% (p < .001) in SMFQ total score with more people (+10%) showing depressive symptoms %during% compared to %before% home confinement. Conclusion The ECLB-COVID19 survey revealed an increased psychosocial strain triggered by the home confinement. To mitigate this high risk of mental disorders and to foster an Active and Healthy Confinement Lifestyle (AHCL), a crisis-oriented interdisciplinary intervention is urgently needed.
Ključne besede: coronavirus, COVID-19, pandemic, public health, restrictions, isolation, home confinement, psychosocial health, psychological consequences, mental health, emotions, wellbeing, physical inactivity
DiRROS - Objavljeno: 18.12.2020; Ogledov: 105; Prenosov: 71
.pdf Celotno besedilo (598,03 KB)

COVID-19 home confinement negatively impacts social participation and life satisfaction : a worldwide multicenter study
Achraf Ammar, Hamdi Chtourou, Omar Boukhris, Khaled Trabelsi, Liwa Masmoudi, Michael Brach, Bassem Bouaziz, Ellen Bentlage, Daniella How, Mona Ahmed, Boštjan Šimunič, Rado Pišot, Saša Pišot, 2020

Povzetek: Public health recommendations and governmental measures during the new coronavirus disease (COVID-19) pandemic have enforced numerous restrictions on daily living including social distancing, isolation, and home confinement. While these measures are imperative to mitigate spreading of COVID-19, the impact of these restrictions on psychosocial health is undefined. Therefore, an international online survey was launched in April 2020 to elucidate the behavioral and lifestyle consequences of COVID-19 restrictions. This report presents the preliminary results from more than one thousand responders on social participation and life satisfaction. Methods: Thirty-five research organizations from Europe, North-Africa, Western Asia, and the Americas promoted the survey through their networks to the general society, in 7 languages (English, German, French, Arabic, Spanish, Portuguese, and Slovenian). Questions were presented in a differential format with questions related to responses "before" and "during" confinement conditions. Results: 1047 participations (54% women) from Asia (36%), Africa (40%), Europe (21%), and others (3%) were included in the analysis. Findings revealed psychosocial strain during the enforced COVID-19 home confinement. Large decreases (p < 0.001) in the amount of social activity through family (%58%), friends/neighbors (%44.9%), or entertainment (%46.7%) were triggered by the enforced confinement. These negative effects on social participation were also associated with lower life satisfaction (%30.5%) during the confinement period. Conversely, the social contact score through digital technologies significantly increased (p < 0.001) during the confinement period with more individuals (+24.8%) being socially connected through digital technology. Conclusion: These preliminary findings elucidate the risk of psychosocial strain during the early COVID-19 home confinement period in 2020. Therefore, in order to mitigate the negative psychosocial effects of home confinement, implementation of national strategies focused on promoting social inclusion through a technology-based solution is strongly suggested.
Ključne besede: coronavirus, COVID-19, pandemic, public health, restrictions, isolation, home confinement, social participation, life satisfaction
DiRROS - Objavljeno: 18.12.2020; Ogledov: 93; Prenosov: 53
.pdf Celotno besedilo (400,20 KB)

Nutritional intake of Slovenian semi-professional handball players
Kaja Teraž, Cécil J. W. Meulenberg, 2019

Povzetek: Aim: Handball is a highly popular team sport, both in Slovenia and globally. The performance during team sport matches is influenced by numerous factors, amongst others there is nutrition whose influence is lesser known, particularly since diet is often uncontrolled. The purpose of this paper is to investigate whether Slovenian handball players, on basis of their anthropometric data and nutritional recommendations, have adequate nutritional intakes of energy and macronutrients. Methods: Two Slovenian handball teams with male and female players (17 males and 9 females) who are part of the 1A national league, were assessed through a 7-day food diary to define their nutritional intake in three different conditions for males (on their training days, match days and days off) and in two conditions for females (on their training days and days off). Basal anthropometric data were measured by standard methods and the body composition by a bioelectrical impedance scale. The results were statistically analysed with IBM SPSS programme, using the repeated measures ANOVA model. Results: Both female and male handball players show insufficient amounts of energy intake, based on the most recent nutritional intake recommendations for team sports. Moreover, the intake of carbohydrates is too low for both female and male handball players, and the intake of fat is too high. Conclusion: The analysis of the initial nutritional intake and the comparison with the recommendation shows that the male handball players do not have an optimal diet with proper intake of both energy and macronutrients. The energy intake is insufficient also in female players. Adjusting the nutritional intake in a way to make it more balanced and tuned to the training schedule, would support a better health and performance.
Ključne besede: energy intake, macronutrients, nutrition, body composition, handball, team sport
DiRROS - Objavljeno: 17.12.2020; Ogledov: 116; Prenosov: 60
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