1. Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornataBarbara Gerič Stare, Nik Susič, Maja Starovič, Saša Širca, 2022, kratki znanstveni prispevek Ključne besede: pathogenicity, plant-parasitic nematodes, nematodes, root-knot nematodes, potato Objavljeno v DiRROS: 27.07.2023; Ogledov: 329; Prenosov: 131 Celotno besedilo (615,48 KB) Gradivo ima več datotek! Več... |
2. Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive responseTjaša Lukan, Anže Županič, Tjaša Mahkovec Povalej, Jacob O. Brunkard, Mirjam Kmetič, Mojca Juteršek, Špela Baebler, Kristina Gruden, 2023, izvirni znanstveni članek Povzetek: Bisphenol A (BPA) is one of the most commonly used substances in the manufacture ofvarious everyday products. Growing concerns about its hazardous properties, including endocrinedisruption and genotoxicity, have led to its gradual replacement by presumably safer analogues inmanufacturing plastics. The widespread use of BPA and, more recently, its analogues has increasedtheir residues in the environment. However, our knowledge of their toxicological profiles is limitedand their combined effects are unknown. In the present study, we investigated the toxic effectscaused by single bisphenols and by the combined exposure of BPA and its two analogues, BPAP andBPC, after short (24-h) and prolonged (96-h) exposure in HepG2 spheroids. The results showed thatBPA did not reduce cell viability in HepG2 spheroids after 24-h exposure. In contrast, BPAP andBPC affected cell viability in HepG2 spheroids. Both binary mixtures (BPA/BPAP and BPA/BPC)decreased cell viability in a dose-dependent manner, but the significant difference was only observedfor the combination of BPA/BPC (both at 40μM). After 96-h exposure, none of the BPs studiedaffected cell viability in HepG2 spheroids. Only the combination of BPA/BPAP decreased cellviability in a dose-dependent manner that was significant for the combination of 4μM BPA and 4μMBPAP. None of the BPs and their binary mixtures studied affected the surface area and growth ofspheroids as measured by planimetry. In addition, all BPs and their binary mixtures studied triggeredoxidative stress, as measured by the production of reactive oxygen species and malondialdehyde,at both exposure times. Overall, the results suggest that it is important to study the effects of BPsas single compounds. It is even more important to study the effects of combined exposures, as thecombined effects may differ from those induced by single compounds. Ključne besede: chloroplast redox state, hypersensitive response (HR)-conferred resistance, immune signaling, live cell imaging, Solanum tuberosum (potato), spatiotemporal analysis, stromules, virus resistance Objavljeno v DiRROS: 13.04.2023; Ogledov: 335; Prenosov: 112 Povezava na datoteko |
3. Analysis of putative apoplastic effectors from the nematode, Globodera rostochiensis, and identification of an expansin-like protein that can induce and suppress host defensesBarbara Gerič Stare, Shawkat Ali, Maxime Magne, Shiyan Chen, Olivier Côte, Natasa Obradovic, Lubna Jamshaid, Xiaohong Wang, Guy Bélair, Peter Moffett, 2015, izvirni znanstveni članek Ključne besede: nematode, expansin-like protein, expansin, potato cyst nematode, potato pest, parasitic nematodes, plant celular function Objavljeno v DiRROS: 06.09.2022; Ogledov: 443; Prenosov: 188 Povezava na celotno besedilo |
4. Late blight resistance conferred by Rpi-Smira2/R8 in potato genotypes in vitro depends on the genetic backgroundEva Blatnik, Marinka Horvat, Sabina Berne, Miha Humar, Peter Dolničar, Vladimir Meglič, 2022, izvirni znanstveni članek Ključne besede: potato, potato genotype, potato R8 genotypes, in vitro infection, late blight, resistant cultivars, oomicete, Sárpo Mira Objavljeno v DiRROS: 01.09.2022; Ogledov: 449; Prenosov: 169 Celotno besedilo (4,07 MB) |
5. Microarray-based uncovering of reference genes for quantitative real-time PCR in potato tuber infected with PVYBarbara Gerič Stare, Aleš Sedlar, Vladimir Meglič, 2021, izvirni znanstveni članek Ključne besede: potato, potato tuber, tuber infection, potato virus Y, real-time PCR, PCR, polymerase chain reaction, reference genes, stored ppotato tubers, pathogens Objavljeno v DiRROS: 31.05.2021; Ogledov: 1280; Prenosov: 285 Celotno besedilo (488,30 KB) |