1. Truffle mycorrhization in a pecan orchardMylla Trisha Mello Souza, Joice Aline Freiberg, Tine Grebenc, Marcelo Aloisio Sulzbacher, Diniz Fronza, Juliano Borela Magalhães, Vanessa da Rosa Gonçalves, Nathalie Caroline Hirt Kessler, Lucas Moura Somavilla, Zaida Ines Antoniolli, 2024, izvirni znanstveni članek Povzetek: The ectomycorrhizal association of Tuber spp. in orchards is essential for truffle production. In this sense, this study aimed to evaluate the ectomycorrhizal association of Tuber aestivum Vittad. and Tuber brumale Vittad. in pecan after five years of cultivation in an orchard. The experiment was conducted in Santa Maria – Rio Grande do Sul, Brazil, in a pecan orchard planted with seedlings inoculated with these two species of truffles. The orchard was characterized in terms of soil physical and chemical properties and soil type. Initially non-inoculated plants, plants inoculated with Tuber aestivum (TA), and plants inoculated with Tuber brumale (TB) were evaluated for the percentage (%) of roots colonized by Tuber spp., other ectomycorrhizae, and non-mycorrhizal roots. A reduction in the colonization of both truffle species was observed five years after transplanting the seedlings to the orchard but they have the potential to maintain the ectomycorrhizal association with pecan seedlings.
Ključne besede: Carya illinoinensis, ectomycorrhiza, truffle, tuber
Objavljeno v DiRROS: 26.03.2024; Ogledov: 90; Prenosov: 55
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2. Description, identification, and growth of Tuber borchii Vittad. mycorrhized Pinus sylvestris L. seedlings on different lime contentsTanja Mrak, Tine Grebenc, Silke Friedrich, Babette Münzenberger, 2024, izvirni znanstveni članek Povzetek: Tuber borchii forms ectomycorrhiza with oaks, hazel, and pines, including Pinus sylvestris. However, its ectomycorrhiza morphotype with P. sylvestris was not comprehensively described so far, and molecular analyses are missing despite a high danger of misidentification of T. borchii ectomycorrhiza with other closely related and less valuable truffle species. We described for the first time the morphology and anatomy of T. borchii-P. sylvestris ectomycorrhiza using differential interference contrast technique and semi-thin sections in combination with molecular confirmation of identity. Color of ectomycorrhiza is reddish to dark brown, and morphotypes are unevenly but densely covered by warts-bearing pin-like cystidia. All layers of the hyphal mantle are pseudoparenchymatous with outer mantle layer formed of epidermoid cells. T. borchii ectomycorrhiza was identified by a molecular comparison with fruitbodies used for inoculation and its respective ectomycorrhizae. T. borchii has a wide ecological amplitude. To get a better insight in mycorrhization requirements, we investigated growth of P. sylvestris and its ectomycorrhiza infection rate with T. borchii in substrate with different lime content. The mycorrhization of P. sylvestris with T. borchii in the mycorrhization substrate and cultivation in greenhouse conditions was successful, with colonization of P. sylvestris varying between 36.5 and 48.1%. There was no significant correlation of mycorrhization to applied lime contents, and consequently to pH in substrate, while the increased levels of lime improved growth of the P. sylvestris seedlings.
Ključne besede: root anatomy, ectomycorrhiza, Limestone content, molecular identification, Pinus sylvestris, Tuber borchii
Objavljeno v DiRROS: 22.01.2024; Ogledov: 189; Prenosov: 92
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3. Microarray-based uncovering of reference genes for quantitative real-time PCR in potato tuber infected with PVYBarbara Gerič Stare, Aleš Sedlar, Vladimir Meglič, 2021, izvirni znanstveni članek Ključne besede: potato, potato tuber, tuber infection, potato virus Y, real-time PCR, PCR, polymerase chain reaction, reference genes, stored ppotato tubers, pathogens
Objavljeno v DiRROS: 31.05.2021; Ogledov: 1306; Prenosov: 294
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4. First report of European truffle ectomycorrhiza in the semi%arid climate of Saudi ArabiaSeema R. Bajaj, Sandesh J. Marathe, Tine Grebenc, Alessandra Zambonelli, Salem Shamekh, 2021, izvirni znanstveni članek Povzetek: Tuber melanosporum Vittad. (Black or Périgord truffle) is a truffle native to the Mediterranean Southern Europe, popular for its unique flavor, and has great economic importance. The present work focused on assessing the possibility of cultivating T. melanosporum associated with Quercus robur L. in the desert climate of Saudi Arabia. The plantation was initiated in November 2018 by planting 271 oak seedlings in the Al-Qassim desert area and checked for survival and ectomycorrhiza development after 1.5 years of plantation maintenance. Amongst the 271 seedlings planted, 243 plants survived two harsh seasons (2019 and 2020), and the randomly selected and tested seedlings were still mycorrhized with T. melanosporum. The mycorrhization level with T. melanosporum was between 5 and 35% of all fine roots, and the share of contaminant ectomycorrhiza was low. In comparison to other areas where T. melanosporum is successfully cultivated, the Al-Qassim desert area has 10%15 °C higher average summer temperatures and a low total annual precipitation, which necessitates regular irrigation of the plantation. This work opens the avenue for an adapted, yet sustainable cultivation of T. melanosporum-inoculated oak tree in a desert climatic condition and introduces new opportunities of the agro-forest business in Saudi Arabia and GCC region.
Ključne besede: Tuber melanosporum, Quercus robur, Desert climate, Truffle cultivation, Ectomycorrhiza
Objavljeno v DiRROS: 26.01.2021; Ogledov: 1064; Prenosov: 280
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5. PCR primers comparisons for a successful Tuber spp. DNA region amplification in routine identificationsTina Unuk Nahberger, Hojka Kraigher, Tine Grebenc, 2020, izvirni znanstveni članek Povzetek: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS re-gion may, due to misidentification, mislabeling or nomen-clature errors in public databases, lead to incorrect or insuf-ficient identification, as is currently a case in the genus Tu b e r. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tu b e r species DNA region amplification. Thereby we (1) compared ampli-fication success for different Tu b e r species using fungal spe-cific primer pair ITS1f and ITS4 and (2) compared amplifi-cation success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aesti-vum. Based on results, Tuber aestivum was one of the most reluctant Tu b e r species in this study and in most cases failed to amplify with the above primer pair. After comparing dif-ferent ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for ampli-fication DNA region of T. ae stiv um as it resulted in high am-plification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus re-sulting in poor or no amplification success. Although prim-er pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tu b e r aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed.
Ključne besede: Tuber spp., ITS region, PCR amplification, ITS primers
Objavljeno v DiRROS: 30.07.2020; Ogledov: 1589; Prenosov: 1173
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6. Karta potencialnih rastišč poletne gomoljike (Tuber aestivum) kot pripomoček pri razvoju gomoljikarstva v SlovenijiJanez Bergant, Andrej Piltaver, Nikica Ogris, Borut Vrščaj, Dušan Jurc, 2014, strokovni članek Ključne besede: zdravje gozdov, gozdovi, gomoljka, Tuber, modeli, karte, potencialna rastišča, gomoljkarstvo
Objavljeno v DiRROS: 28.07.2020; Ogledov: 1671; Prenosov: 834
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