Japanese Knotweed Rhizome Bark Extract Inhibits Live SARS-CoV-2 In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of the Japanese Knotweed Rhizome Bark Extract
2.2. Native SARS-CoV-2 Virus Neutralization Test (VNT)
2.3. Statistical Analysis
3. Results and Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Gorbalenya, A.E.; Baker, S.C.; Baric, R.S.; De Groot, R.J.; Drosten, C.; Gulyaeva, A.A.; Haagmans, B.L.; Lauber, C.; Leontovich, A.M.; Neuman, B.W.; et al. The species Severe acute respiratory syndrome-related coronavirus: Classifying 2019-nCoV and naming it SARS-CoV-2. Nat. Microbiol. 2020, 5, 536–544. [Google Scholar] [CrossRef]
- COVID-19: Epidemiology, Virology, and Prevention. Available online: https://www.uptodate.com/contents/covid-19-epidemiology-virology-and-prevention (accessed on 14 November 2021).
- WHO Director-General’s opening remarks at the media briefing on COVID-19—11 March 2020. World Health Organization (WHO) (Press Release). Available online: https://www.who.int/director-general/speeches/detail/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020 (accessed on 14 November 2021).
- Cui, J.; Li, F.; Shi, Z.L. Origin and evolution of pathogenic coronaviruses. Nat. Rev. Microbiol. 2019, 17, 181–192. [Google Scholar] [CrossRef] [PubMed]
- Machhi, J.; Herskovitz, J.; Senan, A.M.; Dutta, D.; Nath, B.; Oleynikov, M.D.; Blomberg, W.R.; Meigs, D.D.; Hasan, M.; Patel, M.; et al. The natural history, pathobiology, and clinical manifestations of SARS-CoV-2 infections. J. Neuroimmune Pharmacol. 2020, 15, 359–386. [Google Scholar] [CrossRef]
- Pal, M.; Berhanu, G.; Desalegn, C.; Kandi, V. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2): An update. Cureus 2020, 12, e7423. [Google Scholar] [CrossRef] [PubMed]
- Wu, C.; Liu, Y.; Yang, Y.; Zhang, P.; Zhong, W.; Wang, Y.; Wang, Q.; Xu, Y.; Li, M.; Li, X.; et al. Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods. Acta Pharm. Sin. B 2020, 10, 766–788. [Google Scholar] [CrossRef]
- Hoffmann, M.; Kleine-Weber, H.; Schroeder, S.; Krüger, N.; Herrler, T.; Erichsen, S.; Schiergens, T.S.; Herrler, G.; Wu, N.-H.; Nitsche, A.; et al. SARS-CoV-2 Cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell 2020, 181, 271–280. [Google Scholar] [CrossRef]
- Zhou, P.; Yang, X.-L.; Wang, X.-G.; Hu, B.; Zhang, L.; Zhang, W.; Si, H.-R.; Zhu, Y.; Li, B.; Huang, C.-L.; et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020, 579, 270–273. [Google Scholar] [CrossRef]
- Coronavirus Disease (COVID-19): How Is It Transmitted? Available online: https://web.archive.org/web/20201015230546/https://www.who.int/news-room/q-a-detail/coronavirus-disease-covid-19-how-is-it-transmitted (accessed on 14 November 2021).
- Hou, Y.J.; Okuda, K.; Edwards, C.E.; Martinez, D.R.; Asakura, T.; Dinnon, K.H., 3rd; Kato, T.; Lee, R.E.; Yount, B.L.; Mascenik, T.M.; et al. SARS-CoV-2 Reverse genetics reveals a variable infection gradient in the respiratory tract. Cell 2020, 182, 429–446. [Google Scholar] [CrossRef]
- Huang, C.; Wang, Y.; Li, X.; Ren, L.; Zhao, J.; Hu, Y.; Zhang, L.; Fan, G.; Xu, J.; Gu, X.; et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 2020, 395, 497–506. [Google Scholar] [CrossRef]
- Coronavirus (COVID-19), Drugs. Available online: https://www.fda.gov/drugs/emergency-preparedness-drugs/coronavirus-covid-19-drugs (accessed on 12 August 2022).
- COVID-19 Treatments, European Medicines Agency. Available online: https://www.ema.europa.eu/en/human-regulatory/overview/public-health-threats/coronavirus-disease-covid-19/treatments-vaccines/covid-19-treatments (accessed on 12 August 2022).
- Balogh, L. Japanese, Giant and Bohemian knotweed. In The Most Important Invasive Plants in Hungary; Botta-Dukat, Z., Balogh, L., Eds.; HAS Institute of Ecology and Botany: Budapest, Hungary, 2008; pp. 13–33. [Google Scholar]
- Zhang, H.; Li, C.; Kwok, S.-T.; Zhang, Q.-W.; Chan, S.-W. A review of the pharmacological effects of the dried root of Polygonum cuspidatum (Hu Zhang) and its constituents. Evid.-Based Complementary Altern. Med. 2013, 2013, 208349. [Google Scholar] [CrossRef] [Green Version]
- Lin, Y.-W.; Yang, F.-J.; Chen, C.-L.; Lee, W.-T.; Chen, R.-S. Free radical scavenging activity and antiproliferative potential of Polygonum cuspidatum root extracts. J. Nat. Med. 2010, 64, 146–152. [Google Scholar] [CrossRef] [PubMed]
- Hsu, C.-Y.; Chan, Y.-P.; Chang, J. Antioxidant activity of extract from Polygonum cuspidatum. Biol. Res. 2007, 40, 13–21. [Google Scholar] [CrossRef] [PubMed]
- Pogačnik, L.; Rogelj, A.; Ulrih, N.P. Chemiluminescence method for evaluation of antioxidant capacities of different invasive knotweed species. Anal. Lett. 2015, 49, 350–363. [Google Scholar] [CrossRef]
- Lachowicz, S.; Oszmianski, J. Profile of bioactive compounds in the morphological parts of wild Fallopia japonica (Houtt) and Fallopia sachalinensis (F. Schmidt) and their antioxidative activity. Molecules 2019, 24, 1436. [Google Scholar] [CrossRef]
- Ardelean, F.; Moacă, E.A.; Păcurariu, C.; Antal, D.S.; Dehelean, C.; Toma, C.-C.; Drăgan, S. Invasive Polygonum cuspidatum: Physico-chemical analysis of a plant extract with pharmaceutical potential. Studia Univ. Vasile Goldis Arad Ser. Stiintele Vietii 2016, 26, 415–421. [Google Scholar]
- Kurita, S.; Kashiwagi, T.; Ebisu, T.; Shimamura, T.; Ukeda, H. Content of resveratrol and glycoside and its contribution to the antioxidative capacity of Polygonum cuspidatum (Itadori) harvested in Kochi. Biosci. Biotechnol. Biochem. 2014, 78, 499–502. [Google Scholar] [CrossRef]
- Chan, C.-L.; Gan, R.-Y.; Corke, H. The phenolic composition and antioxidant capacity of soluble and bound extracts in selected dietary spices and medicinal herbs. Int. J. Food Sci. Technol. 2016, 51, 565–573. [Google Scholar] [CrossRef]
- Nawrot-Hadzik, I.; Ślusarczyk, S.; Granica, S.; Hadzik, J.; Matkowski, A. Phytochemical diversity in rhizomes of three Reynoutria species and their antioxidant activity correlations elucidated by LC-ESI-MS/MS analysis. Molecules 2019, 24, 1136. [Google Scholar] [CrossRef]
- Zhang, C.; Zhang, X.; Zhang, Y.; Xu, Q.; Xiao, H.; Liang, X. Analysis of estrogenic compounds in Polygonum cuspidatum by bioassay and high performance liquid chromatography. J. Ethnopharmacol. 2006, 105, 223–228. [Google Scholar] [CrossRef]
- Xue, Y.; Liang, J. Screening of bioactive compounds in rhizoma Polygoni cuspidati with hepatocyte membranes by HPLC and LC-MS. J. Sep. Sci. 2014, 37, 250–256. [Google Scholar] [CrossRef]
- Fan, P.; Zhang, T.; Hostettmann, K. Anti-inflammatory activity of the invasive neophyte Polygonum cuspidatum Sieb. and Zucc. (Polygonaceae) and the chemical comparison of the invasive and native varieties with regard to resveratrol. J. Tradit. Complementary Med. 2013, 3, 182–187. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Shan, B.; Cai, Y.-Z.; Brooks, J.D.; Corke, H. Antibacterial properties of Polygonum cuspidatum roots and their major bioactive constituents. Food Chem. 2008, 109, 530–537. [Google Scholar] [CrossRef]
- Yiu, C.-Y.; Chen, S.-Y.; Huang, C.-W.; Yeh, D.-B.; Lin, T.-P. Inhibitory effects of Polygonum cuspidatum on the Epstein-Barr virus lytic cycle. J. Food Drug Anal. 2011, 19, 107–113. [Google Scholar] [CrossRef]
- Chang, J.-S.; Liu, H.-W.; Wang, K.-C.; Chen, M.-C.; Chiang, L.-C.; Hua, Y.-C.; Lin, C.-C. Ethanol extract of Polygonum cuspidatum inhibits hepatitis B virus in a stable HBV-producing cell line. Antivir. Res. 2005, 66, 29–34. [Google Scholar] [CrossRef]
- Kuo, Y.-T.; Liu, C.-H.; Li, J.-W.; Lin, C.-J.; Jassey, A.; Wu, H.-N.; Perng, G.C.; Yen, M.-H.; Lin, L.T. Identification of the phytobioactive Polygonum cuspidatum as an antiviral source for restricting dengue virus entry. Sci. Rep. 2020, 10, 16378. [Google Scholar] [CrossRef] [PubMed]
- Yan, J.I.; Hong-Xia, W.A.; Zuo-Yi, B.A.; Guan-Fu, Z.H. Evaluation of antiviral effect of Polygonum cuspidatum water extract with a model of murine acquired immunodeficiency syndrome. Virol. Sin. 1998, 13, 311. [Google Scholar]
- Lin, H.-W.; Sun, M.-X.; Wang, Y.-H.; Yang, L.-M.; Yang, Y.-R.; Huang, N.; Xuan, L.-J.; Xu, Y.-M.; Bai, D.-L.; Zheng, Y.-T.; et al. Anti-HIV activities of the compounds isolated from Polygonum cuspidatum and Polygonum multiflorum. Planta Med. 2010, 76, 889–892. [Google Scholar] [CrossRef]
- Lin, C.J.; Lin, H.J.; Chen, T.H.; Hsu, Y.A.; Liu, C.S.; Hwang, G.Y.; Wan, L. Polygonum cuspidatum and its active components inhibit replication of the influenza virus through toll-like receptor 9-induced interferon beta expression. PLoS ONE 2015, 10, e0117602. [Google Scholar] [CrossRef]
- Chen, K.-T.; Zhou, W.-L.; Liu, J.-W.; Zu, M.; He, Z.N.; Du, G.H.; Chen, W.W.; Liu, A.L. Active neuraminidase constituents of Polygonum cuspidatum against influenza A(H1N1) influenza virus. Zhongguo Zhong Yao Za Zhi 2012, 37, 3068–3073. [Google Scholar] [CrossRef]
- Wang, Z.; Huang, T.; Guo, S.; Wang, R. Effects of emodin extracted from Rhizoma Polygoni Cuspidati in treating HSV-1 cutaneous infection in guinea pigs. J. Anhui Tradit. Chin. Med. Coll. 2003, 22, 36–38. [Google Scholar]
- Yiu, C.-Y.; Chen, S.-Y.; Chang, L.-K.; Chiu, Y.-F.; Lin, T.-P. Inhibitory effects of resveratrol on the Epstein-Barr virus lytic cycle. Molecules 2010, 15, 7115–7124. [Google Scholar] [CrossRef] [PubMed]
- Evers, D.L.; Wang, X.; Huong, S.-M.; Huang, D.Y.; Huang, E.-S. 3,4′,5-Trihydroxy-trans-stilbene (resveratrol) inhibits human cytomegalovirus replication and virus-induced cellular signaling. Antivir. Res. 2004, 63, 85–95. [Google Scholar] [CrossRef]
- Docherty, J.J.; Fu, M.M.; Stiffler, B.S.; Limperos, R.J.; Pokabla, C.M.; DeLucia, A.L. Resveratrol inhibition of herpes simplex virus replication. Antivir. Res. 1999, 43, 145–155. [Google Scholar] [CrossRef]
- Docherty, J.J.; Sweet, T.J.; Bailey, E.; Faith, S.A.; Booth, T. Resveratrol inhibition of varicella-zoster virus replication in vitro. Antivir. Res. 2006, 72, 171–177. [Google Scholar] [CrossRef] [PubMed]
- Kapadia, G.J.; Azuine, M.A.; Tokuda, H.; Takasaki, M.; Mukainaka, T.; Konoshima, T.; Nishino, H. Chemopreventive effect of resveratrol, sesamol, sesame oil and sunflower oil in the Epstein-Barr virus early antigen activation assay and the mouse skin two-stage carcinogenesis. Pharmacol. Res. 2002, 45, 499–505. [Google Scholar] [CrossRef] [PubMed]
- Zhang, H.-S.; Zhou, Y.; Wu, M.-R.; Zhou, H.-S.; Xu, F. Resveratrol inhibited Tat-induced HIV-1 LTR transactivation via NAD+-dependent SIRT1 activity. Life Sci. 2009, 85, 484–489. [Google Scholar] [CrossRef] [PubMed]
- Yiu, C.-Y.; Chen, S.-Y.; Yang, T.-H.; Chang, C.-J.; Yeh, D.-B.; Chen, Y.-J.; Lin, T.-P. Inhibition of Epstein-Barr virus lytic cycle by an ethyl acetate subfraction separated from Polygonum cuspidatum root and its major component, emodin. Molecules 2014, 19, 1258–1272. [Google Scholar] [CrossRef]
- Shuang-Suo, D.; Zhengguo, Z.; Yunru, C.; Xin, Z.; Baofeng, W.; Lichao, Y.; Yan’an, C. Inhibition of the replication of hepatitis B virus in vitro by emodin. Med. Sci. Monit. 2006, 12, 302–306. [Google Scholar]
- Liu, Z.; Wei, F.; Chen, L.-J.; Xiong, H.-R.; Liu, Y.-Y.; Luo, F.; Hou, W.; Xiao, H.; Yang, Z.-Q. In vitro and in vivo studies of the inhibitory effects of emodin isolated from Polygonum cuspidatum on Coxsakievirus B4. Molecules 2013, 18, 11842–11858. [Google Scholar] [CrossRef]
- Yang, Y.; Islam, M.S.; Wang, J.; Li, Y.; Chen, X. Traditional Chinese medicine in the treatment of patients infected with 2019-new coronavirus (SARS-CoV-2): A review and perspective. Int. J. Biol. Sci. 2020, 16, 1708–1717. [Google Scholar] [CrossRef]
- Ho, T.-Y.; Wu, S.-L.; Chen, J.-C.; Li, C.-C.; Hsiang, C.-Y. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction. Antivir. Res. 2007, 74, 92–101. [Google Scholar] [CrossRef] [PubMed]
- Schwarz, S.; Wang, K.; Yu, W.J.; Sun, B.; Schwarz, W. Emodin inhibits current through SARS-associated coronavirus 3a protein. Antivir. Res. 2011, 90, 64–69. [Google Scholar] [CrossRef] [PubMed]
- Lin, S.-C.; Ho, C.-T.; Chuo, W.-H.; Li, S.; Wang, T.T.; Lin, C.-C. Effective inhibition of MERS-CoV infection by resveratrol. BMC Infect. Dis. 2017, 17, 144. [Google Scholar] [CrossRef] [PubMed]
- Nawrot-Hadzik, I.; Zmudzinski, M.; Matkowski, A.; Preissner, R.; Kęsik-Brodacka, M.; Hadzik, J.; Drag, M.; Abel, R. Reynoutria rhizomes as a natural source of SARS-CoV-2 Mpro inhibitors-molecular docking and in vitro study. Pharmaceuticals 2021, 14, 742. [Google Scholar] [CrossRef]
- Lin, S.; Wang, X.; Tang, R.W.; Lee, H.C.; Chan, H.H.; Choi, S.S.A.; Dong, T.T.; Leung, K.W.; Webb, S.E.; Miller, A.L.; et al. The extracts of Polygonum cuspidatum root and rhizome block the entry of SARS-CoV-2 wild-type and omicron pseudotyped viruses via inhibition of the S-protein and 3CL protease. Molecules 2022, 27, 3806. [Google Scholar] [CrossRef]
- Jug, U.; Naumoska, K.; Vovk, I. (−)-Epicatechin—An important contributor to the antioxidant activity of Japanese knotweed rhizome bark extract as determined by antioxidant activity-guided fractionation. Antioxidants 2021, 10, 133. [Google Scholar] [CrossRef]
- Grodowska, K.; Parczewski, A. Organic solvents in the pharmaceutical industry. Acta Pol. Pharm. 2010, 67, 3–12. Available online: https://www.ptfarm.pl/wydawnictwa/czasopisma/acta-poloniae-pharmaceutica/110/-/12992 (accessed on 10 September 2020).
- Lu, Y.; Wang, J.; Li, Q.; Hu, H.; Lu, J.; Chen, Z. Advances in neutralization assays for SARS-CoV-2. Scand. J. Immunol. 2021, 94, e13088. [Google Scholar] [CrossRef]
- Jug, U.; Glavnik, V.; Vovk, I.; Makuc, D.; Naumoska, K. Off-line multidimensional high performance thin-layer chromatography for fractionation of Japanese knotweed rhizome bark extract and isolation of flavan-3-ols, proanthocyanidins and anthraquinones. J. Chromatogr. A 2021, 1637, 461802. [Google Scholar] [CrossRef]
- Cos, P.; De Bruyne, T.; Hermans, N.; Apers, S.; Vanden Berge, D.; Vlietinck, A.J. Proanthocyanidins in health care: Current and new trends. Curr. Med. Chem. 2004, 11, 1345–1359. [Google Scholar] [CrossRef]
- Fujii, Y.; Suhara, Y.; Sukikara, Y.; Teshima, T.; Hirota, Y.; Yoshimura, K.; Osakabe, N. Elucidation of the interaction between flavan-3-ols and bovine serum albumin and its effect on their in-vitro cytotoxicity. Molecules 2019, 24, 3667. [Google Scholar] [CrossRef] [PubMed]
- Xia, L.; Shi, Y.; Su, J.; Friedemann, T.; Tao, Z.; Lu, Y.; Ling, Y.; Lv, Y.; Zhao, R.; Geng, Z.; et al. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19: Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine 2021, 85, 153390. [Google Scholar] [CrossRef] [PubMed]
- Zhang, W.; Xie, Q.; Xu, X.; Sun, S.; Fan, T.; Wu, X.; Qu, Y.; Che, J.; Huang, T.; Li, H.; et al. Baidu Jieduan granules, traditional Chinese medicine, in the treatment of moderate coronavirus disease-2019 (COVID-19): Study protocol for an open-label, randomized controlled clinical trial. Trials 2021, 22, 476. [Google Scholar] [CrossRef] [PubMed]
- Zhao, J.; Pan, B.; Xia, Y.; Liu, L. Network pharmacology-based analysis reveals the putative action mechanism of Polygonum cuspidatum against COVID-19. Int. J. Clin. Exp. Med. 2021, 14, 1852–1863. [Google Scholar]
- Xu, H.; Li, J.; Song, S.; Xiao, Z.; Chen, X.; Huang, B.; Sun, M.; Su, G.; Zhou, D.; Wang, G.; et al. Effective inhibition of coronavirus replication by Polygonum cuspidatum. Front. Biosci. 2021, 26, 789–798. [Google Scholar] [CrossRef]
- Yu, M.X.; Song, X.; Ma, X.Q.; Hao, C.X.; Huang, J.J.; Yang, W.H. Investigation into molecular mechanisms and high-frequency core TCM for pulmonary fibrosis secondary to COVID-19 based on network pharmacology and data mining. Ann. Palliat. Med. 2021, 10, 3960–3975. [Google Scholar] [CrossRef]
- Wang, M.; Qin, K.; Zhai, X. Combined network pharmacology, molecular docking, and experimental verification approach to investigate the potential mechanisms of polydatin against COVID-19. Nat. Prod. Commun. 2022, 17, 1934578X221095352. [Google Scholar] [CrossRef]
- Pasquereau, S.; Nehme, Z.; Haidar Ahmad, S.; Daouad, F.; Van Assche, J.; Wallet, C.; Schwartz, C.; Rohr, O.; Morot-Bizot, S.; Herbein, G. Resveratrol inhibits HCoV-229E and SARS-CoV-2 Coronavirus replication in vitro. Viruses 2021, 13, 354. [Google Scholar] [CrossRef]
- Wiwanitkit, V. Polydatin and COVID-19. Clin. Nutr. [CrossRef]
- Naumoska, K.; Jug, U.; Kõrge, K.; Oberlintner, A.; Golob, M.; Novak, U.; Vovk, I.; Likozar, B. Antioxidant and antimicrobial biofoil based on chitosan and Japanese knotweed (Fallopia japonica, Houtt.) rhizome bark extract. Antioxidants 2022, 11, 1200. [Google Scholar] [CrossRef]
- Van Doremalen, N.; Bushmaker, T.; Morris, D.H.; Holbrook, M.G.; Gamble, A.; Williamson, B.N.; Tamin, A.; Harcourt, J.L.; Thornburg, N.J.; Gerber, S.I.; et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N. Engl. J. Med. 2020, 382, 1564–1567. [Google Scholar] [CrossRef] [PubMed]
- Why the Coronavirus Has Been So Successful. Available online: https://www.theatlantic.com/science/archive/2020/03/biography-new-coronavirus/608338/ (accessed on 14 November 2021).
- Meyers, C.; Kass, R.; Goldenberg, D.; Milici, J.; Alam, S.; Robison, R. Ethanol and isopropanol inactivation of human coronavirus on hard surfaces. J. Hosp. Infect. 2021, 107, 45–49. [Google Scholar] [CrossRef] [PubMed]
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Jug, U.; Naumoska, K.; Malovrh, T. Japanese Knotweed Rhizome Bark Extract Inhibits Live SARS-CoV-2 In Vitro. Bioengineering 2022, 9, 429. https://doi.org/10.3390/bioengineering9090429
Jug U, Naumoska K, Malovrh T. Japanese Knotweed Rhizome Bark Extract Inhibits Live SARS-CoV-2 In Vitro. Bioengineering. 2022; 9(9):429. https://doi.org/10.3390/bioengineering9090429
Chicago/Turabian StyleJug, Urška, Katerina Naumoska, and Tadej Malovrh. 2022. "Japanese Knotweed Rhizome Bark Extract Inhibits Live SARS-CoV-2 In Vitro" Bioengineering 9, no. 9: 429. https://doi.org/10.3390/bioengineering9090429